小發(fā)夾RNA（英語：short hairpin RNA，縮寫shRNA）是一種形成急轉(zhuǎn)彎（hairpin turn）結(jié)構(gòu)的RNA序列，可以經(jīng)由RNA干擾（RNAi）使基因表現(xiàn)沉默化。shRNA可利用載體導(dǎo)入細胞當(dāng)中，并借由U6啟動子來確保shRNA的表現(xiàn)。另外，shRNA可經(jīng)由切割轉(zhuǎn)變成為siRNA
參考文獻
- McIntyre G, Fanning G. Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnol.. 2006, 6: 1. PMID 16396676.
- Harper S, Staber P, He X, Eliason S, Martins I, Mao Q, Yang L, Kotin R, Paulson H, Davidson B. RNA interference improves motor and neuropathological abnormalities in a Huntington's disease mouse model. Proc. Natl. Acad. Sci. U.S.A.. 2005, 102 (16): 5820-5. PMID 15811941.
- Nielsen M, Pedersen F, Kjems J. Molecular strategies to inhibit HIV-1 replication. Retrovirology. 2005, 2: 10. PMID 15715913.
- Paddison P, Caudy A, Bernstein E, Hannon G, Conklin D. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev.. 2002, 16 (8): 948-58. PMID 11959843.
- Cao W, Hunter R, Strnatka D, McQueen C, Erickson R. DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response. J. Appl. Genet.. 2005, 46 (2): 217-25. PMID 15876690.
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